These notes provide a comprehensive overview of Biophysics course, covering the fundamentals of radiation–matter interaction in proteins. The notes examine absorption spectroscopy—including the derivation of the Lambert–Beer law and the principles of electronic and vibrational transitions—and explores light scattering techniques, such as Rayleigh and Raman scattering, the Clausius–Mossotti relationship, and dynamic light scattering to determine molecular size and diffusion. Advanced topics include Förster Resonance Energy Transfer (FRET) for measuring intramolecular distances and protein–protein interactions, as well as Electron Paramagnetic Resonance (EPR) to probe spin dynamics, relaxation processes, and magnetic interactions, with special emphasis on spin labeling. These notes offer a robust foundation in both theoretical and experimental aspects of modern biophysical techniques.
Università della Calabria
Fisica
Biophysics And Human Physiology